Context: Plasmodium falciparum malaria is the leading cause of death and morbidity in the Democratic Republic of Congo. Despite the existing of several control methods, cases of malaria are still increasing.

Objectives: To measure the level of knowledge of health care providers on malaria and ways to control it, and to determine attitudes and practices to perform when malaria occurs.

Methodology: This was a facility-based cross-sectional study involving 467 health care providers in xx health facilities. All care providers who were on duty on the day of the survey were included. Data collection consisted in a face-to-face interview using a study questionnaire. Two knowledge scores were created. The analyses were carried out using EPI INFO software version 3.5.1

Results: Of the 467, providers 70% of these providers were retrained on malaria control measures. Only 4% of care providers know the signs of seriousness. The use of LLIN and cleaning up of the environment are the best-known preventive measures, while IPT-SP (p≤0.001) and even less the indoor spraying (p = NS) of insecticide are less well known. At-risk groups are better known by care providers in referral hospitals than in health posts and centers (84.6% vs 67.1 %, p< 0.001). At the knowledge level, more than 66% care providers from private sector have an inadequate state of knowledge compared to 40% of providers from public sector (p <0.001). Also, only 17% care providers from private sector versus 43.8% from public sector have above-average knowledge (p <0.001). The referral is considered as measure of care in almost 30% of cases in the public sector against only 4.1% in the private sector (p< 0.001).