Background: De Quervain’s tenosynovitis is a painful condition affecting the wrist and thumb, commonly associated with occupations requiring repetitive wrist and thumb movements, such as tailoring. Understanding its prevalence and awareness in high-risk occupational groups is essential for planning preventive strategies.

Aim: To determine the prevalence of De Quervain’s tenosynovitis and the level of awareness regarding this condition among female tailors in Latur city.

Materials and Methods: An observational cross-sectional study was conducted among female tailors in Latur city. A total of 114 participants were screened, of which 109 met the inclusion criteria. All eligible subjects underwent the Finkelstein test to identify clinical suspicion of De Quervain’s tenosynovitis. Awareness and knowledge regarding the condition were assessed using a structured questionnaire consisting of seven questions. Data were analyzed using descriptive statistics and Chi-square tests with SPSS software.

Results: Of the 109 participants assessed, 14 (12.8%) had a positive Finkelstein test, while 95 (87.2%) were negative, indicating a low prevalence of De Quervain’s tenosynovitis in this population. The mean age of the participants was 31.43 ± 5.29 years (range: 21–48 years). Awareness of the condition was extremely low: 106 (97.2%) participants had never heard of De Quervain’s tenosynovitis, and only 3 (2.8%) reported any prior knowledge. Only 2.8% had ever consulted a physician for wrist or thumb pain. Age was significantly higher among those with a positive Finkelstein test (34.43 ± 7.69 years) compared to those with a negative test (31.09 ± 4.84 years; p = 0.029).