(112) swab samples of burn and wounds were collected from patients incoming to the Specialized Center for Burns and Plastic Wounds in Mosul City/ Iraq, from September 2022 to the end of January 2023, for different ages ranging (18 months to 55 years) and for both sexes. After culturing (112) wound swabs, traditional biochemical methods and the (API 20E) system for investigation, as well as the Vitek-2 compact system were used to diagnosis the bacterial isolates.

A molecular diagnostic of Citrobacter species was carried out based on the outcomes of the PCR method by the 16SrRNA gene. This involved sequential analysis of the nitrogenous bases to complete the investigative genes of the bacterial isolates.