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VOL. 11, ISSUE 1 (2024)
Concentration dependence of the influence of sodium nitrite on the oxidative degradation of hem during hydrogen peroxide oxidation of hemoglobin
Authors
T M Huseynov, S M Rahmanova, H A Abiyev
Abstract
The study examined the
concentration dependence of the inhibition of oxidative degradation of heme
hemoglobin (Hb) induced by a bolus dose of hydrogen peroxide (H2O2)
with sodium nitrite (NaNO2). Oxidative degradation of heme caused by
H2O2 is accompanied by the formation of fluorescent heme
breakdown products. And nitrites can inhibit this process, despite the fact
that they themselves are capable of oxidizing Hb, depriving it of its ability
to transfer O2, and creating hypoxia and have a toxic effect. It was
possible to establish the lower concentration limit of the inhibitory effect
(NO2), which starts from 0.5 mM and higher according to the
quenching of characteristic fluorescence (λexcitation = 321 nm (313 nm),
λemission = 465 nm (460 nm)
and λexcitation = 437 nm, λemission = 520 nm) The
effect of maximum inhibition is achieved at concentrations of 2.0 mM-5.0 mM.
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Pages:1-5
How to cite this article:
T M Huseynov, S M Rahmanova, H A Abiyev "Concentration dependence of the influence of sodium nitrite on the oxidative degradation of hem during hydrogen peroxide oxidation of hemoglobin". International Journal of Multidisciplinary Research and Development, Vol 11, Issue 1, 2024, Pages 1-5
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