The present study aimed to finding the relationship between plasmid content with resistance of antibiotic and heavy metal salts in Staphylococcus aureus and Pseudomonas aeruginosa isolated of pathogenic and environmental sources.

The results of antibiotics sensitivity tests showed that all studied isolates had multidrug resistance to these antibiotics and all isolates showed absolute resistance to Amoxicillin, Ampicillin and Streptomycin. The isolates varied in their tolerance to different concentrations of studied heavy metals salts, some isolates showed absolute resistance (concentration 1.6 mg / ml) towards most of these studied minerals (5 out of 6 minerals) and all isolates had absolute resistance to Lead acetate (CH₃Co₂)₂ Pb. The isolates were also varied in their plasmid content (1-2 bundles) depending on their source of isolation.