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VOL. 10, ISSUE 12 (2023)
Impact of Azadirachtin on in vitro pollen germination of Catharanthus roseus (L.) G. Don. and Clitoria ternatea L
Authors
Saloni Bahri, Madhu Bajaj, Renuka Agrawal, Somdutta Sinha Roy, Yamini Sharma, Mishthi Khurana
Abstract
Several kinds of fungicides, insecticides and pesticides are used in
crop fields to protect the plants from attack by different types of pathogens.
Many of these synthetic chemicals have toxic effects on plants as demonstrated
by experiments performed on germination of pollen grains under in vitro
conditions. Pollen have been found to be a convenient system to assess the
cytotoxic effects of chemicals. They can be easily cultured in a specific
germination medium containing the chemical whose toxicity is to be assessed. In
the present investigation, effect of a bioinsecticide Azadirachtin on pollen
germination and growth of pollen tube of two medicinally important plant
species, viz. Clitoria ternatea L. and Catharanthus roseus (L.)
G. Don. under in vitro conditions has been studied. Pollen grains were
cultured in germination media containing four dilutions of Azadirachtin i.e.,
1:1, 1:2, 1:4 and 1:8. The observations clearly indicate a decrease in percent
pollen germination and inhibition of pollen tube growth. Further studies should
be carried out to determine the dose of bioinsecticide required for a particular
crop and its time of application to combat its negative impact.
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Pages:42-47
How to cite this article:
Saloni Bahri, Madhu Bajaj, Renuka Agrawal, Somdutta Sinha Roy, Yamini Sharma, Mishthi Khurana "Impact of Azadirachtin on <i>in vitro</i> pollen germination of <i>Catharanthus roseus</i> (L.) G. Don. and <i>Clitoria ternatea</i> L". International Journal of Multidisciplinary Research and Development, Vol 10, Issue 12, 2023, Pages 42-47
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