The integration of school and enterprise culture is an effective way to highlight the characteristics of vocational education and realize the seamless connection between school teaching content and enterprise post requirements. It is also an important part of the socialization process of students. Various higher vocational colleges and enterprises have taken a series of measures to achieve the integration of school-enterprise culture, and achieved some results. However, with the deep integration of school-enterprise culture, the problems of school culture and enterprise culture in the aspects of values, systems, norms of behavior and so on are constantly exposed, which affects the play of vocational education functions and the development of enterprises. Based on the above problems, this paper discusses the specific countermeasures for the deep integration of college and enterprise culture in higher vocational colleges.