An in vitro cultivation protocol was developed for Salvia fruiticosa Mill, a species threatened by over collection due to its importance as a medicinal plant in Al-Jabal Al-Akhdar area, Libya. The aim of this study was to develop an efficient micropropagation method for. fruiticosa, that would contribute to the conservation and commercial propagation of this species. Effect of different plant growth regulators onCallus induction, explants (leaves, stems and roots) cultured on MS media supplemented with: Dichlorophenoxyacetic acid (2, 4-D), Benzylaminopurine (BAP) and Naphthaleneacetic acid (NAA). Interaction between media composition and types of explants (leaves, stems and roots) on callus induction %, fresh /dry weights and water content showed that leaf explants responded with higher significant levels (70%, 2.841, 0.186 g/jar and 60%) respectively then roots (62%, 1.321, 0.034 and 33%) and lastly, stems (22%, 0.123, 0.031 and 16%) when cultured on medium received only 2,4- D.