Cercospora leaf spot disease cause heavy economic losses in Indian spinach. The aim of this research is to evaluate the efficacy of botanicals and biological agents namely Neem leaf extract (T2) and Biskatali leaf extract (T3) were used 1:1 w/v as seed treatment along with another two treatments like Trichocompost (T1) and Cowdung (T4) which were used as soil treatment before sowing (2 kg/m2). Two treatments of leaf extracts were also used with concentration 1:2 (w/v) as spray solution in field condition. Investigation in vivo field experiment showed that Trichocompost remarkably reducing the intencity and disease severity. Biskatali leaf extract and Cowdung also gave satisfactory result to control Cercospora leaf spot of Indian spinach compared to neem leaf extracts. The results of the present studies suggested that, use of biological agents and botanical treatments especially Trichocompost and Biskatali leaf extract has ability to minimize Cercospora leaf spot disease and improve the growth of Indian spinach.