Aiming to develop more efficiency and environmental friendly methods than those are available to control Colletotrichum capsici, which causes anthracnose of chilli. The study investigate to appropriate natural management a search for fungal bio-agents, plants and SM of endophytic fungi that able to produce substances active against such pathogen was carried out. In my first experiment three fungal bio-agents were collected from Plant Disease Clinic, PLP, PSTU. Among them the significantly highest inhibition 62.14 % was achieved by Trichoderma sp. Thus, the second experiment six plant parts were selected, that were prepared and submitted to in vitro assays with that fungus. The best results were obtained with the extracts prepared from Mehgoni seeds (Switeenia mahagoni), which inhibited C. capsici 83.372% at 40% concentration. In my third experiment, secondary metabolites (SM) of two endophytic fungi were isolated and tested against C. capsici and the highest mycelial growth inhibition was found 91.525% by SM of Pestalotia sp. Concluding, all the experiments were presented antifungal potential for new bioactive substances to control of C. capsici, especially Trichoderma sp., Mehgoni seeds and Pestalotia sp. highly inhibited the mycelial growth.