In any breeding plan genetic diversity is significant importance. Genotypic and phenotypic diversity allows a breeder to select varieties having manifold traits concurrently. In current study, to found out the genetic diversity among 66 accessions of Gossipium hirsutum, we used thirty-one simple sequence repeat (SSR) markers. These markers produced total 138 loci and an average locus per primer was 4.4. Polymorphism information content (PIC) ranged from 0.27 to 0.92 with an average value 0.59. BNL-2709, NAU-5418, J-274 SSR markers gave maximum polymorphism. Gene diversity ranged from 0.32 to 0.92 and average was 0.64. BNL-2709, NAU-5418, J-274 gave maximum gene diversity. The range of pair-wise genetic estimation based on Nei 1973 was from 0.09 to 0.93. A dendrogram was produced by using the UPGMA analysis which grouped the 66 accessions into two main clusters. One cluster comprises 40 accessions while other cluster constitutes 26 accessions. We also check diversity on the basis of phenotypic traits that allows a breeder to select varieties with interesting genotypic phenotypic characters at the same time. Correlation analysis among phenotypic traits gave positive correlation that ranged from 0.00 to 0.78 while negative correlation ranged from -0.001 to -0.363. Principle component analysis (PCA) was performed which showed variation on the basis of traits among varieties and also show the major contributor in the variation. PCA analysis produced 6PCs component contributing to 86% variability, PC1 is responsible for maximum variation with 18.22. Minimum variation was contributed by PC14 that was 1.103. Cluster analysis divided the 66 accessions in to 5 clusters on basis of various phenotypic traits. Tarzan-5 genotype falls in separate cluster representing its diversity from other accessions. In cluster analysis, genotypes in cluster 1 showed maximum value of plant height (97.1), boll per plant (37.0). Cluster 2 manifested maximum value of plant height (274.3), boll per plant (28.1). Cluster 3 showed maximum value of plant height (151.6), boll per plant (32.0). Cluster 4 manifested maximum value of plant height (101.6), seed per boll (26.3). Cluster 5 showed maximum value of plant height (127.0), seed per boll (24.2).
Anum Shaheen, Syed Bilal Hussain, Syed Aun Muhammad, Muhammad Zubair, Tahir Naqqash. Molecular and phylogenetic study of CLCuD tolerant cotton cultivars by using microsatellite markers. International Journal of Multidisciplinary Research and Development, Volume 8, Issue 9, 2021, Pages 25-30