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VOL. 8, ISSUE 9 (2021)
Determination of genomic diversity of upland cotton (Gossipium hirsutum) genotypes using ssr markers
Authors
Samreen Sarwar, Syed Bilal Hussain, Syed Aun Muhammad, Muhammad Zubair, Tahir Naqqash
Abstract
Genetic diversity is prerequisite for any breeding plan. Genetic diversity along with phenotypic diversity provides the chance to select the genotypes with multiple good traits simultaneously. To find out the genetic diversity among 45 accessions of Gossypium hirstum, 44 Simple sequences repeats (SSR) and expressed sequence tag SSRs (EST-SSR) were used (from BNL, DPL, JESPER and NAU series). Total of 222 alleles were produced with an average of 4.9 alleles per primer. Polymorphism Information Content (PIC) value was ranged from 0.16 to 0.19 with a mean value of 0.76. Pair-wise genetic estimation based on Nei 1973 ranged from 0.50 to 0.96, with an average 0.79. In bootstrap cluster analysis maximum accession falls into a single cluster indicating low genetic diversity. Positive correlation among phenotypic traits ranged from 0.001 to 0.99 while, negative correlation from -0.004 to -026. Principle components analysis (PCA) analyses generate the 7 PCs components contributing 71.1%. Maximum variation contributed by PC1 is 14.3. Cluster analysis grouped the 45 accession into 4 clusters. Twenty nine out of 45 genotypes fall in a single cluster. Phenotipycally CIM-707 showed maximum diversity while, genotypically CIM-707 showed little resemblance with CIM-600, CIM-132 and CIM-622. Low genetic diversity was observed among both genetic and phenotypic as maximum accessions shears the same group. This study helps for selecting diverse accessions with multiple phenotypic traits simultaneously. This also suggests furthering elaborating the molecular genetic diversity by using more SSR marker to improve the resolution of upland cotton for good breeding plans.
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Pages:16-21
How to cite this article:
Samreen Sarwar, Syed Bilal Hussain, Syed Aun Muhammad, Muhammad Zubair, Tahir Naqqash "Determination of genomic diversity of upland cotton <em>(Gossipium hirsutum) </em>genotypes using ssr markers ". International Journal of Multidisciplinary Research and Development, Vol 8, Issue 9, 2021, Pages 16-21
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