Identification of rotavirus G-P genotypes among Iraqi children in Diyala province by multiplex semi-nested RT-PCR
Ali R Hameed, Bakri Y Mohamed, Adam D Abakar, Karim S Ali
Background: Rotavirus is a much more popular cause of viral diarrhea. and it has several genotypes. Objectives: The aim of this study is to molecular identification of the Rotavirus G and P genotypes by detecting the VP7 and VP4 genes and to evaluate the relationship between the clinical presentation and rotavirus genotypes.Material and Methods: This study was conducted on children with diarrhea under (5) years at Batool Hospital in Diyala province during 2019-2020. RNA extraction of 142 stool samples for children with rotavirus virus and G-P genotypes identified by Multiplex semi-nested RT-PCR.Results: The results showed, prevalent G-genotypes were G1, G4, G2, G9, G1/G2 and G1/G4 at 37.32%, 29.58%, 21.83%, 2.11% and 0.70% respectively, whereas prevalent P-genotypes were P, P, P and P at 56.34% 13.38%, 8.45%, and 4.23% respectively. The G and P-type combinations were PG4, PG1, PG1, PG1, PG2, PG2, PG4, PG4 and PG1/G4 at 24.65%,23.94%, 8.45%, 4.93%, 4.23%. 4.23%, 2.82%. 2.11% and 0.70% respectively. PG1 the more predominant G-P genotype combination in children with fever and severe dehydration, while P G4 most common in children with vomiting and some dehydration. Our study did not demonstrate any relationship between genotypes and clinical presentation.Conclusion: There are different Rotavirus genotypes in Diyala province. The current study predicts that the Rotavirus vaccine used in Iraq is still protective for humans. On the other hand, there is no assortment between the human and animal rotavirus strains and our rotavirus strains in this study are similar to Russian strains.
Ali R Hameed, Bakri Y Mohamed, Adam D Abakar, Karim S Ali. Identification of rotavirus G-P genotypes among Iraqi children in Diyala province by multiplex semi-nested RT-PCR. International Journal of Multidisciplinary Research and Development, Volume 8, Issue 7, 2021, Pages 34-40