Color is an important factor for determining the ornamental fish price in the world market. Use of carotenoid source beetroot (Beta vulgaris) in the powder form can be changed the skin color of three spot gourami. Beetroot are a great source of fiber, folate (vitaminB9), manganese, potassium, iron and vitamin C. Carotenoid present in Beetroot is 1.9mg/100g of the sample. Altogether 12 fish were randomly selected into 3 group into three tanks. The experimental feeds were prepared with 30% protein the basic ingredients such as GNC and maize and the natural source of carotenoid, beetroot are randomly added in experimental diet 0%, 5% and 10% for three tanks and give the feed for fishes 4% body weight for a period 60 days. In the control tank no other change was determined, but in the experimental tank colour, weight and length is different from one to another tank. In control tank color was no changed and the growth is poor in compare to other experimental tanks. The best result colour and growth was appear in 10%beetroot feed then 5% beetroot feed.