Leishmniasis one of the recognized disease in tropical countries including Afghanistan, this research determine the antileishmanial activity of Nicotiana tabaccum methanol extracts against promastigotes of L. tropica, which is the causative agent of Nangarhar province Afghanistan. Different concentrations (100 µgmL-1, 50µgmL-1, 25µgmL-1) of Nicotina Tabacum extraction were screened to investigate leishmanial activates against Nicotiana tabaccum methanol extracts a long three times (24, 48, 72) Hours. The Percentage inhibition of Promastigote More Then (22%) After 24 And 48 Hours incubation, While After 72hours increase (36%). Standard division at the Highest Concentration of 100 µgmL-1 was (0, 094624) While at the Lowest Concentration of 25µgmL-1 was (0.014266) % after 24, 48 And 72 Hours.