Background & Objective: Nature of the work is an accidental discovery of new Haemoflagellate in human blood sample. Methods: Finger prick blood sample collected stained with JSB I and JSB II for malaria microscopy, declared negative for malaria parasite. Cross checked for malaria parasite. Results: Unidentified haemoflagellate detected in blood sample exhibited new morphological structure than known. Interpretation & Conclusions: Known uni-flagellate protozoa in humans are Trypanosoma and Leishmania. Trypanosoma observed in mastigote form with undulating membrane while Leishmania remain oval amastigote form in humans. The newly identified haemoflagellate has cup shaped morphology with a single flagellum. It resembles the characteristics of family Trypanosomatidae. The life cycle, vector, host and disease caused by this haemoflagellate are unknown.