This study was carried out for developing high yielding cotton genotypes equipped with attractive fiber superiority parameters under drought condition. Five upland cotton accessions were screened out as drought tolerant and three as drought susceptible on the basis of root shoot parameters. The results of root shoot screening experiment were verified in field screening on the basis of agronomical and physiological parameters. These eight genotypes were grown in glass house conditions and crossed in line ×tester mating design. During the next cotton season, these fifteen cotton hybrids and parents were sown in field conditions. Analysis of variance revealed existence of significance amongst the accessions for all the parameters i.e., Plant Height(PH), number of bolls (NB), boll weight (BW), seed cotton yield (SCY), seed index (SI), ginning out-turn (GOT), fiber length (FL), fiber fineness (FF), fiber strength (FS). Further, the line i.e., MS-64 and tester i.e., BH-176 had better yield performance under water stress. The cross combinations viz., GS-444 x MPS-11, and COOKER-315 x Cyto-62 were also recognized as best superior for yield contributing traits. These combinations might be helpful for fresh germplasm development on large scale under water stress condition.