High-performance liquid chromatographic analysis of the extracts of ginger, hibiscus and colves herbal plant
Amina Aboubakr Bala Mohamed, Dr. Mohamed Elmukhtar Abd Aziz, Ahmed Elsadg Mohamed Saeed
A high performance liquid chromatographic method was carred out to estimte niacine, ascorbic acid and gallic acid in ginger, hibiscus and colves samples using aqueous extract; a very simple method was performed. A C18 column was used with a gradient elution of mobile phase at a flow rate of 0.38 cm3/min. The results obtained showed the presence of niacine and gallic acid, in all herbal samples. Ginger gave high concentration of niacine (11.48 ppm), then hibiscus (1.04 ppm) and colves (0.525ppm). Ginger gave a high concentration of gallic acid (56 ppm), then hibiscus (12.05 ppm)and colves(2. 265 ppm). However, The concentration of vitamin C was not detected in the three herbal samples because extraction method used might not be suitable for its extraction.