A novel, simple, specific and economic UV spectrophotometric method was developed using water as solvent to estimate niacin content in ginger. The λ-max of niacin was found to be 262 nm. Linearity in the concentration range of 5-30 μg/cm³ was found to be exhibiting good correlation coefficient (R2=0.9955). The developed method was validated statistically to demonstrate linearity, accuracy, precision, LOD and LO. The results of the study proved the applicability of the present method in routine analysis of niacin in ginger.