Objective:To determine the effect of kinesio taping on ankle joint pain, balance and functional performance in patients with lateral ankle instability. Material and Methods:The study included 30 subjects with age was ranged from 18 to 35 years; they were divided into two groups: experimental group A: This group Consisted of 15 patients with lateral ankle sprain treated by selected balance training program in form of three sessions per week for four weeks, in addition to taping the ankle joint with adhesive elastic kinesio tape. Control group B: This group Consisted of 15 patients with lateral ankle sprain treated by selected balance training program in form of three sessions per week for four weeks, the same balance training as group (A) but without the use of kinesio tape to the ankle joint. The investigations were conducted at balance unit, in sport medicine specialized center in Nasr city. Measurements were conducted before starting the treatment as a first record and at the end of 4 weeks of treatment as second record. Exclusive criteria were subjects who had other co morbidities which affect results. Results: when comparing the two groups (A and B) before treatment, the mean ± SD values were 2.79 ± 1.05 and 2.46 ± 0.92 respectively which indicated no significant improvement (p= 0.365), while comparing the two groups after four weeks of treatment, the mean ± SD values were 1.33 ± 0.36 and 1.9 ± 0.69 respectively which indicated a significant improvement (p= 0.011) in favor of group (A) (MD= 0.57) and % of improvement was 21.42 %. Conclusion: it was concluded that kinesio taping had improvement on pain, balance and functional performance in patient with lateral ankle sprain.