Background: Many studies in the general population have shown a link between Helicobacter pylori infection and iron-deficiency, often resulting in iron-deficient anemia. Despite the high prevalence of iron deficiency in hemodialysis patients, no studies have been performed in this population. Objective: To evaluate the role of Helicobacter pylori infection in the appearance of anemia and the iron requirements in our hemodialysis population. Material and methods: After excluding patients with severe pathology and short life expectancy and those with blood losses secondary to other causes, 90 patients were included. Iron requirements and anemia were determined by serum iron, ferritin, and hematocrit values; and by transfusion, erythropoietin and iron requirements. The diagnosis of Helicobacter pylori status was established by fecal antigen of Helicobacter. Results: Prevalence of Helicobacter pylori infection was 55.6%. No significant differences Between patients infected or not by Helicobacter pylori were found in any of the variables analyzed, serum iron in H. pylori positive group was lower than in H. pylori negative group (86 ± 17.5 vs. 87 ± 18.2 ug/dl) but it was not statistically significant (p=0.960) Biochemical studies revealed that mean TIBC in H. pylori positive group was higher than in H. pylori negative group (321.1 ± 95.0 vs. 295.1±101.0 ug/dl) but it was. not statistically significant (p=0.232), Regarding Transferrin saturation, it was lower in H. pylori positive group than in H. pylori negative group (26.79 % ± 18.42 % vs. 29.83% ± 18. 01 %) but it was not statistically significant (p=0.596) that mean serum ferritin in H. pylori positive group was lower than in H. pylori negative group (284.8 ± 60,5 vs. 301.4 ± 50.1 ng/dl) but it was not statistically significant (p=0.069). Conclusion: H. pylori was found in 55.56% of studied patient, no relation between dyspeptic symptom and H. pylori infection was found, H. pylori infection does not have any influence on anemia severity or iron deficiency in these patients.