A field experiment was carried out to evaluate the effect of different levels of mascagnite solution on some bio-chemical parameters such as: proline, Leghaemoglobin, chlorophyll, nitrogen, phosphorous, protein, and nitrate and nitrite reductase enzymes activity in lentil crop. The experiments were conducted in the research farm of the Botany Department of C.C.S. University, Meerut in Rabi season (2017-18). Mascagnite, a source of nitrogen and sulfur nutrients has the potential agronomic benefits in increasing crop production over other commonly used N and S fertilizers. 1.5 % of ammonium sulphate was found beneficial for the lentil crop productivity as compared to others remaining 0%, 0.5%, 1.0% treatments. 1.5% of mascagnite solution had the better potential to reduce the stress conditions and enhanced the biochemical pathways to provide essential elements such as nitrogen or sulfur.