This study is a research and development (R&D) which refers to 4-D development model. The objective of this study is to produce a valid, practical, and effective scientific approach-based learning module in Plantae concept. The study employed descriptive qualitative and descriptive quantitative approaches. The prototype 1 was validated by the material experts. The prototype 11 was tested by using the experiment design of pre-test and post-test only to examine the practicality level and effectiveness of Biology learning module developed at Senior High School (SMA) 2 Sengkang Wajo Regency South Sulawesi Indonesia in class X of academic year 2014/2015. The result of the study revealed that the scientific approach based learning module was confirmed as valid. The practical level valued for the implementation of learning which used scientific approach-based learning module was in the high category. The effectiveness of module valued from the teacher's ability in managing the learning was in a good category, the result of students' response was 94.61% which had the good response and students' learning result improved from 35.65 to 84.35 with completeness percentage by 94.11%. Thus, the conclusion of the study was the scientific approach-based learning module developed in Plantae concept was valid, practical, and effective to be used in Biology lesson.