Purpose: To evaluate the level of serum angiopoietin-1 in the patients with diabetic nephropathy in Upper Egypt and to study the relation between it’s level and the severity of renal dysfunction in those patients. Design: Cross section design. Methods: sixty diabetic patients selected from out patients clinic of internal medicine department, al azhar assuit univerisity hospital and divided into three groups as regards to the level of albumminuria, and twenty healthy individuals were being selected as acontrol group. Results: Reported 24 months Study of Serum Angiopoietin-1 in diabetic nephropathy Patients in Upper Egypt and to study the relation between it’s level and the severity of renal dysfunction in those patients there is : Ang1 was significantly lower in Normo-albuminuria group (4824.27±1363.3 vs 3186.55±2470.8) pg/ml respectively, Macro-albuminuria group (4824.27±1363.3 vs 1188.06±755.3) pg/ml respectively and Micro-albuminuria group (4824.27±1363.3 vs 3109.08±556.9) pg/ml respectively. It was significantly higher in Normo-albuminuria group compared to Macro-albuminuria group (3186.55±2470.8 vs 1188.06±755.3) pg/ml respectively. Also, it was significantly higher in Micro-albuminuria compared to Macro-albuminuria group (3109.08±556.9 vs 1188.06±755.3) pg/ml respectively. There was significant inverse correlation between serum angiopoietin-1 and both serum creatinine (r = -0.375) and urine albumin creatinine ratio (r = -0.547), while there was significant direct correlation between serum angiopoien-1 and eGFR (r=0.363). There was no significant correlation with age, BMI, and HbA1c% (p>0.05). Conclusion: Decrease of serum Angiopoietin-1 level in patients with diabetic nephropathy may play a big role in pathogenisis of the disease especially because serum angiopoietin-1 is decreasing more with the progression of the proteinuria. Hence this conclusion may implement angiopoietin-1 in experimental treatment studies to prevent or improve diabetic nephropathy or to study the cause effect relationship of serum angiopoietin-1 and diabetic nephropathy to use angiopoietin-1 as a diagnostic tool for diabetic nephropathy disease.