A laboratory experiment was conducted at Department of Seed Science and Technology, UAS, GKVK, Bangalore, during 2013-2014 to study the “Effect of genotypes, seed treatment chemicals and packaging materials on seed quality of onion (Alliums cepa L.). The two genotypes of onion hybrid Arka Kirtiman and variety Arka Kalyan seeds were treated with Captan@2g/kg, Thiram@2 g/kg and untreated seeds served as control. The treated seeds were packed in cloth bag, polythene 700 gauge bag, super grain bag, poly pouch and aluminium foil and subsequently stored for a period of ten months under ambient conditions of Bangalore. The results revealed that onion seeds treated with Captan@2g/kg packed in 700 gauge polythene and aluminium foil. The seeds are stored under ambient condition maintained higher seed quality parameters with minimum seed certification standards up to 10 months.