The objectives to assess the knowledge and practice of post-natal mothers regarding lactation management before and after intervention and to determine the effectiveness of structured teaching programme. To associate the finding with demographic variable. A quasi experimental one group pretest post-test design was used. Total 60 post-natal mothers were selected by non-probability purposive sampling technique .Data collection was done by using questionnaire and observation check list. Demographic data shows that 70 % post-natal mothers were from age group of 18 to 25 of years, 53% mothers had no formal education, 70% post-natal mothers lived in rural area, 92% mothers had undergone LSCS and 88% of mothers were house maker. In pretest 71.6% post-natal mothers had poor knowledge were as in post test 91.6% post-natal mothers had good knowledge about lactation management. Mean score of pretest was 6.5 which had increased to 21.85 in posttest and p-valve for it was 0.00 which suggest improvement in knowledge. 83% mothers had poor practices before intervention, 70% had good practices after intervention. Mean score of before intervention observation was 1.3 which had increased to 8.6 in after intervention and p-valve for it was 0.00 which suggest improvement in practices.