Pollen Grains are one of the biological components present in air. These are produced by the male reproductive part of the plants. Inspite of being small in size they play an important role in various studies forming separate disciplines, such as, areopalynology, archaeopalynology, forensic palynology, palaeopalynology, copropalynology, melissopalynology, etc. Therefore the identification of these tiny components becomes a necessity. In present investigation pollen morphology of 52 tree species of Allahabad was studied.