In the present investigation, plant parts of Ageratum conyzoïdes and Chromolaena odorata were collected to analyse the antifungal activity against Cercospora spp. isolated from banana, mango and safou fruits in Dschang, Cameroon. Antifungal activity was determined in vitro following Agar-well diffusion method using different concentrations of 1.25 ; 2.5 ; 5 and 10 mg/ml for ethanol extracts and 5 ; 10 ; 15 ; 20 mg/ml for aqueous extracts. Ethanolic extracts of Ageratum conyzoïdes completely inhibited the growth of C. capsici and C. mangiferae at 5 and 10 mg/ml respectively. With the ethanolic extracts of Chromolaena odorata, 100% inhibition was observed at 5 mg/ml against C. mangiferae and C. capsici. Aqueous extracts of Chromolaena odorata and Ageratum conyzoïdes completely inhibited C. musae, C. mangiferae and C. capsici at 20 mg/ml. In vivo test showed that fruits inoculated with C. musae and C. capsici and treated with the ethanolic extracts of A. conyzoïdes and C. odorata did not develop any lesion 5 days after inoculation. Ethanolic extracts possess biofungicidal potential, which can suitably be exploited to control fruit rots.