The researcher will attempt to throw light on the influence of organized Physical Education training programme, that prevailing in the training colleges of our country, on trainee females on perspectives of fitness. The subjects of the present study were 18 female students aged 22 through 24 years. They were undergoing physical education training course (2014-15) in the Department of Physical Education Kalyani University, West Bengal. Physical fitness was considered as criteria to assess the influence of organized physical education programme on trainee-females. The total duration of the training course was ten months (July 1^st to May 6th next year). The structure of the training programme was two and half hour’s morning activity session, two hours afternoon activity session and four hours theoretical session. Moreover there were a number of outdoor education session which includes camping, picnic, officiating, tour, social service etc. Physical fitness in general, of the trainee females was improved significantly following participation in the organized physical education training programme. Performance in all the six tests of physical fitness showed significant improvement during post test than that of pre test. The magnitude of improvement was maximum in the sit up test (38.50%) followed by flexed arm hang test (29.20%) 50 yard dash (26.36%), shuttle run (11.40%) 600 yard Run and Walk (10.97%) and was minimum in standing Broad jump (4.06%).