Fish, in Bangladesh where malnutrition remains a significant development challenge, is an irreplaceable animal-source food in the diet of millions. Biochemical-composition, mineral-profile and microbial-quality of three fish species namely Gudusia chapra (chapila), Xenetodon cancila (kaika) and Mastacembelus pancalus (baim) inhabiting in Meghna River, Bangladesh was studied in order to evaluate their nutritional values. It was investigated that mean moisture, protein, fat, ash and TVB-N value were 76.41±0.02%, 10.53±%, 11.62±%, 1.53±% and 2.40± mgN/100g in chapila fish and 77.67±%, 14.27±%, 4.66±%, 2.94±% and 4.92±mgN/100g in kaika fish whereas 77.21±%, 15.17±%, 6.13±%, 1.72±% and 3.16± mgN/100g in baim fish respectively. The Total viable counts were in acceptable limits. The pattern of mineral composition in chapila, kaika and baim fishes, are in the order; Ca>Mg>Fe>Zn>Cu>Mn. The data showed that these species of fishes are rich in nutrient value that are not less than larger fish and meet the requirement for human nutritional needs.