Cranio-cervical flexion test (CCFT) was one among the method for evaluating the Deep Neck Flexor activity and is reliable in asymptomatic individuals. Studies demonstrated that patient with chronic neck pain had poor ability to perform the CCFT when compared with asymptomatic subjects. The objective of the study was to test the intra-rater reliability of the cranio-cervical flexion test in subject with cervical spondylosis. Thirty subjects diagnosed with cervical spondylosis were recruited for the study. All the patient need to performed standardized CCFT protocol. Five progressive stages were achieved with 10 seconds hold at each stage followed by 30 seconds rest and score was note. After the 24 hours again test was performed and the score was done and tests score has been analyzed. The Intra rater reliability coefficient was 0.504 (95% Confidence interval, 0.73) which was statistically significant (p-value <0.002). Results showed that there is moderate intra-rater reliability in subject with cervical spondylosis. Cranio-cervical flexion test can be used as a measurement tool to rule out the Deep neck flexor activity in cervical spondylosis.