Tomato (Lycopersicon esculentum Mill.) is economically the most important and popular fresh market vegetables throughout the world. It occupies number one position in its nutrient contribution to human diet. Fusarium wilt is known as one of the most devastating diseases of tomato worldwide. It is an important soil inhabiting fungi, and is known to be phylogenetically diverse. Most strains assigned to this species are saprophytic or non-pathogenic. Pathogenic fungi of the genus fusarium that are the causal agents of tomato wilt cause root and basal stem deterioration and result in the wilting of vegetable plants. Browning of the vascular tissue is strong evidence of fusarium wilt. The experiment was conducted under in-vitro and field culture conditions to observe the effect of bio-agents, botanicals and fungicide against Fusarium oxysporum. For the in-vitro studies five treatments were studied. Therefore, the Maximum percentage of inhibition was recorded in Trichoderma harzianum 74.53%, Ashoka 28.82%, Psidium guajava 23.61%, Aloe-vera 20.48%, Lantana camera 3.47%, followed by carbendazim 100%. The inhibition and effect of bio agent (Trichoderma harzianum) and leaf extracts against tested pathogen was due to competition and preventing the mycelia growth of the pathogen, which is very valuable and riskless in eco-friend, animals and human health.