Despite numerous published studies which focused on the toxicity of different chemicals on aquatic organisms. Among the various aquatics, amphibians are good candidates for aquatic Nano toxicology since they have the potential of being bio indicators and also are of great importance for investigation of the pollution transference between terrestrial and aquatic ecosystems. In different studies difference in toxicity was also seen due to change in the size time, age and condition of test organisms. Zebra fish is extensively studied model in the Ag-NPs toxicological studies. The toxicity indicators may include, drop in heart rate, hatching delay and higher mortality rate. Amphibians play both the role of prey and top predators in trophic chains based on their habitat and life stage.