Vegetables can become contaminated with pathogenic organism during growth, harvest, postharvest handling or distribution. The microbial (Total Coliforms, Faecal coliforms, Staphylococcus aureus, Listeria spp., Pseudomonas spp, Salmonella spp) quality of vegetables (Spinach, Cucumber, Ladyfinger and Ridge guard) irrigated with waste water in around Yamuna river of Allahabad district investigated. Total coliform and faecal coliform were observed positive in all vegetables, The ANOVA tables appended with appendix showed Non-significant result of Staphylococus aureus for site one (Mahewa Path) but in site two(Arail Ghat), significant result was obtained for Staphylococcus aureus. Non-significant result of Listeria spp for site one but in site two significant results was obtained for Listeria spp. There was significant result of salmonella spp in site one similarly in site two. The quality of the waters and the irrigated vegetables exceeded standard microbiological limits. We recommend that the government enforce strict rules and legislation on adequate treatment of wastewater and effluents before discharge to the environment.