Typhoid remains one of the challenging waterborne diseases in Africa, especially in Kenya due to lack of clean drinking water in rural and slum areas. This study involved evaluation of herbal medicine for typhoid treatment using plants used by herbalists in Embu and Mbeere regions of Kenya The evaluation was done by carrying out bioassay studies to determine the activity of botanical microbial against disease causing agents using the Disk Diffusion method. The aqueous extracts were tested for their inhibitory activity against one selected strain of Salmonella typhi bacteria. All the selected plant extracts investigated exhibited activity against Salmonella typhi with inhibition zone diameters ranging from 4.4 – 18.5 mm. Aloe secundiflora gave the highest inhibition zones against S. typhi followed by Vernonia brachycalyx and Terminalia brownii. It was confirmed that Carrisa edulis, Aloe secundiflora and Tithonia diversifolia were also active against Salmonella typhi. These findings provided a scientific basis for the use of the tested herbal plant extracts in the treatment of typhoid and other bacterial diseases by herbalists in Eastern Kenya.