The purpose of the study was to determine the effect of SAQ training on selected bio-motor variables such as speed, agility and quickness among female Kho-Kho players. In this study 20 female players from GDC for Women, Baramulla were selected as subjects and their age ranged from 18 - 23 years. These subjects were divided randomly into two groups of ten each (n=10). Group-1 underwent SAQ exercises followed by varied intensities for three alternative days per week for six weeks and Group-2 acted as control group who did not participate in any training during the training period other than their daily schedule in the curriculum. The data pertaining to this study were collected by administering 50 meter dash test, Shuttle Run Test and Hop test and was statistically analyzed by t-test and to find out the significant difference on adjusted posttest among the groups, analysis of co-variance (ANCOVA) was used. The level of significance to test the hypothesis was kept at 0.05. On the basis of the findings it was concluded that there was significant improvement on speed, agility and quickness due to the effect of SAQ training among female Kho-Kho players when compared to control group and also significant difference towards improving the selected bio-motor variables such as speed, agility and quickness among female Kho-Kho players than control group.