The aim of the present study is to assess the effects of Pranayama on Breath-Holding Capacity, Cardio-Vascular Endurance & Reaction Time of high school students in Punjab. Two hundred boy’s age group of 13 to 16 years from Govt. High School, Karala, Punjab and S.B.W.S.M.P. School, Banur, Punjab were selected as the research subjects. The Pranayama training duration was of 10-weeks. The subjects were divided into two groups as experimental (Group A) and control (Group B). The experimental group underwent Pranayama training for 10-weeks and control group did not receive the Pranayama training. The ‘t’ test was used to compare pre and post-training values. After10-weeks Pranayama training, there was a significant (P< 0.001) difference between pre and post-testing of experimental group for the breath-holding capacity (pre = 35.89±1.55, post=36.92±1.57), cardio-vascular endurance (pre= 1710.27±50.73, post 1785.51±78.24) and reaction time (pre = 24.81±0.40, post 23.55±0.43) as well as control group for the breath-holding capacity (pre = 34.28±1.01, post = 34.27±1.02), cardio-vascular endurance (pre = 1580.94±13.62, post = 1498.17±62.78) and reaction time (pre = 25.90±0.50, post = 25.83±0.52). The experimental group had a significant improvement on Breath-Holding Capacity, Cardio-Vascular Endurance & Reaction Time than the control group.