The high cost of importing fish feeds into Ghana has necessitated the search for possibly cheaper substitute feed with high nutritional value. The proximate composition of housefly maggots (Musca domestica) cultured on animal blood and digester, poultry waste and brewer’s spent (pito waste) was analyzed. There was significant (p<0.05) difference in the proximate composition of maggots from the different substrates. Poultry waste seems to be a promising substrate to be used in maggots’ production for maggot meal. However, any of the understudied substrates have the tendency to be used for the production of maggot meal to feed fish since they all have competitive nutrient values.