Macrobenthic and bacterial fauna play an important role in maintaining the food chain and for exchange of nutrients in the aquatic ecosystem. The presently investigated subtropical perennial pond of Jammu situated near Birpur area. The study was carried to analyse water quality of pond in relation to its physico-chemical status and biological characteristics. Bottom sediment sample from which bacterial colonies and macrobenthic invertebrates were isolated had also been analysed for various physico-chemical parameters. Macrobenthic invertebrates were represented by Chironomus chironomus, Chironomus pupae, Pentaneura sp., Melanoides tuberculata, Physa sp., Tubifex tubifex, Branchiura sp. and Dero digitata. From the bacterial study, bacterial colonies identified were Gram +ve Bacilli, Gram -ve Bacilli, Gram +ve Cocci and Gram -ve Cocci. Comparative analysis between Bacterial colonies inhabiting sediments and water the results revealed fewer bacteria in Pond water as compared to sediment sample. Presence of few pollution indicator organisms infers that the water is getting polluted and approaching towards eutrophication.