International Journal of Multidisciplinary Research and Development

International Journal of Multidisciplinary Research and Development


International Journal of Multidisciplinary Research and Development
International Journal of Multidisciplinary Research and Development
Vol. 2, Issue 11 (2015)

In vitro Antioxidant Activity, Phytochemical analysis and Cytotoxicity of Ethanolic leaves Extract of Moringa oleifera lam


Mohammed A Abass, Ahmed S Kabbashi, Mohammed I Garbi

Interest in natural products as a source for innovation in drug discovery and agrochemicals is still growing worldwide. Natural products, whose immense diversity has been appreciated for many years, may become in a rich source of novel chemical structures. The present study was conducted to investigate the in-vitro antioxidant (DPPH assay), phytochemical screening and cytotoxicity (MTT-assay) of ethanol extract of Moringa oleifera (leaves). The methanol extract of M. oleifera (leaves) was tested for antioxidant screening for their free radical scavenging properties using 2.2Di (4-tert-octylphenyl)-1-picryl-hydrazyl (DPPH), while propyl galate was used as standard antioxidant, phytochemical screening and cytotoxicity(MTT assay) with different concentration (500 ppm, 250 ppm and 125 ppm) and compare triton-100 (the reference control). The ethanol extract of M. oleifera (leaves) antioxidant activity was (75 0.01 RSA%) in comparison to the control of propyl galate levels (88 0.07RSA%), Preliminary phytochemical screening of the leaves of M. oleifera revealed that the plant contain triterpenes, alkaloids, tannins, glycosides, coumarins saponins and flavonoids. Negative results were recorded for alkaloids. And in addition cytotoxicity (MTT assay) with different concentration (500, 250 and 125 ppm) in comparison to triton-x100 (the reference control) which verified the safety of the examined extract with an IC50 less 100 ?g/ml. Hence, the results obtained in the present study indicate that M. oleifera have promising antioxidant indicates that the plant could be promising agent in scavenging free radicals and treating diseases related to free radical reactions.
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How to cite this article:
Mohammed A Abass, Ahmed S Kabbashi, Mohammed I Garbi. In vitro Antioxidant Activity, Phytochemical analysis and Cytotoxicity of Ethanolic leaves Extract of Moringa oleifera lam. International Journal of Multidisciplinary Research and Development, Volume 2, Issue 11, 2015, Pages 73-77
International Journal of Multidisciplinary Research and Development International Journal of Multidisciplinary Research and Development