The objective of the present study was to study the effects of different concentration of sodium fluoride on different yield and its bioaccumulation in barley (Hordeum vulgare).In a pot experiment and field observation, a barley was irrigated with Double distilled water (Control) and different levels of sodium fluoride ( 3, 6, 9, 12, 15 and 18ppm). The experiment was carried out for the entire life cycle of 105 days of this barley variety. Plants were harvested after 35 days (pre-flowering), 75 days (peak-flowering) and 105 days (post-flowering) of sowing of seeds. Bioaccumulation studies of fluoride in plant parts revealed maximum accumulation in leaves parts (9.948mg/kg) and minimum in Grain (6.302mg/kg) in plants treated with 18ppm NaF in pot experiment. But field observation of sodium fluoride in crop plant parts revealed maximum (16.758mg/kg) accumulation in leaves and minimum in Grain (9.351mg/kg) in plants treated well, tube well water in field experiment. Results showed that the use of groundwater containing high fluoride content for irrigating Barley crop plant may be tolerating to its biological accumulation in different plant parts.