Aristolochia bracteolata Linn. (family- Aristolochiaceae) commonly called as Hukka-Bel and worm killer in English). It is used in traditional medicines as a gastric stimulant and in the treatment of cancer, lung inflammation, dysentery and snakebites. In the indigenous system of medicine, the plant was antipyretic & anti-inflammatory agents. The plant contain Aristolochic acid, has many medicinal properties in various disease condition. The Phytochemical showing revealed the presence of alkaloids, triterpenoids, steroids and sterols, flavonoids, tannins and phenolic compounds and cardio glycosides. Maximum bud break of the explant was obtained on MS medium supplemented with 2.0 mg l-1 of benzyl adenine (BA) + additives. Multiple shoots produced from MS+0.25 mgl-1 each of BAP, Kinetin and IAA. After 10-15 days of cultures. Amplified shoots from meristematic explants. In vitro multiplied shoots pulse treated with root hormone the rooted plantlets were hardened in green house with relieve and transplanted into black polybags containing a 1:1:1 (v/v/v) ratio of garden soil, organic manure and sand.