Antioxidants are helpful in the defense mechanism of body against different pathogens. The use of plant derived antioxidants is helpful against many degenerative diseases like Parkinson, Alzheimer and Cancer. This review gives an overlook to the different methods used to determine antioxidant capacity of different antioxidants. The explanation of analytical performances and principles of different methods used for the determination of antioxidant capacity involve chromatography, spectrometry and electrochemical techniques are discussed in detailed.