This paper investigated the effect of collaborative learning strategy and photo-visual literacy on the academic performance of students in chemistry. The study adopted a quasi-experimental design approach. The target groups for the study were senior secondary school students in Ikwerre Local Government Area of Rivers State. The study sampled 200 SSII students, randomly selected from two senior secondary schools in in Port Harcourt metropolis. The two schools were randomly placed as experimental/treatment and control groups each with 100 students. The experimental/treatment group was further divided into two groups A and B. The dependent variable was measured through a self-constructed 20-item performance test used as a pre-test and post-test. The collaboration strategy and photo-visual strategy were used on the experimental group while lecture method was used for the control group. Data analysis was done using mean, standard deviation, t-test, ANOVA and scheffe multiple comparism test. The findings showed that collaboration strategy was more effective than photo-visual literacy. Hence there was a significant difference in the effectiveness of collaboration and photo-visual literacy. It is recommended that student should be encouraged to read collaboratively instead of individually in other to enhance academic performance.