Background: Diabetic foot infection is one of the dreaded complications of diabetes mellitus, several studies have been undertaken in the past which have shown high variations among them and are contradictory. The aim of the present study is to know the bacteriological profile and antibiotic susceptibility pattern of such infections which will help in proper management of these patients. Materials and methods: Samples were collected from 110 patients during April 2018 to May 2019, using sterile swabs and were processed as per standard procedures and antibiotic susceptibility testing was done as per CLSI 2019 guidelines. Result: Samples were taken from 110 patients from whom 140 bacterial isolates were obtained. Among 110 patients, males 62 (56.36%) were affected more by diabetic foot infection as compared to female 48 (44%). Among 110 patients most common age group affected in both sexes is 41-50 years (41%) followed by 51-60 years (36.4%), 31-40 years (13%) and 61-70years (10%). No cases were reported in patients between age group of 10-20 years and age group more than 70 years. Patients with one pathogen isolated were 42(32.2%) whereas patients with more than one pathogen isolated were 68(62.0%). Total bacterial isolates obtained from 110 patients were 140 out of which 125 (89.3%) gram negative bacteria were isolated while 15(11%) were gram positive bacteria. Most common bacteria isolated is Pseudomonas aeruginosa 50(36%) followed by Proteus vulgaris 30(21.42%), Klebsiella pneumoniae 15(21%), E. coli 18(13%), Streptococcus spp 15(11%) and Acinetobacter baumanii 6(4.3%). Among staphylococcus species (n=15), Staphylococcus aureus were 9(60%), CONS 4(27%), MRSA 2(13.33%). Among gram negative bacteria high sensitivity was seen for Imipenem 113(94%) followed by Amikacin, Piperacillin-tazobactum 85% respectively, Gentamicin and ceftriaxone 75%, ciprofloxacin 60% and Cotrimoxazole 50%. Among Gram negative bacteria (n=125), 38(30%) isolates were ESBL producers while 13((10%) isolates were MBL producer. Among gram positive bacteria (n=15), MRSA 2(13.33) were seen. Conclusion: Diabetic foot infection is a medical emergency and it is associated with life threatening complication. Proper management of the patient with appropriate antibiotics will help in better patient outcome.