The purpose of the study was to evaluate the effectiveness of Instructional Strategy based on E-learning on Vocabulary Acquisition in English among Primary School Students. The Experimental Method with the Pretest-Posttest Nonequivalent-Groups Design was used for the study. The technique of simple random sampling was employed to collect data. The data was analyzed using Arithmetic Mean, Standard Deviation, Critical Ratio (t test) and Analysis of Covariance (F test). The findings of the study showed that the Instructional Strategy based on E-learning is very effective in enhancing Vocabulary Acquisition in English among Primary School Students.