International Journal of Multidisciplinary Research and Development

Authors
Poorna Chandrika S, Sabarinathan D, Vinothini S, Preethi K

Abstract
Bioremediation being a problem solver for many different environmental and other major hazards, they play an important and cost effective role in the biodecaffeination process. Twenty strains were isolated from coffee husk dumped soil, among that one strain could grow on the medium supplemented with 15g/L caffeine and could effectively degrade up to 2.5 g/L of caffeine in the liquid media as a sole source of carbon and nitrogen. Morphological and biochemical characteristics identified the organism as Enterobacter aerogenes. The degradation of caffeine was authenticated by growth curve and UV- Spectrometry analysis. The enzyme responsible for decaffeination was partially purified and identified using SDS- PAGE.