International Journal of Multidisciplinary Research and Development

International Journal of Multidisciplinary Research and Development


International Journal of Multidisciplinary Research and Development
International Journal of Multidisciplinary Research and Development
Vol. 1, Issue 5 (2014)

Mathematical modeling predicts physical parameters which are pertinently affecting the 2D- and 3D- culture microenvironments


Yeh Chen, Xian-Yu Yeh, Chiung-Chi Peng, Kuna-Chou Chen, Robert Y. Peng

The 2-D disc and 3-D RWV cultures showed quite a big discrepancy in a diversity of biological responses. The reasons are still poorly understood. We hypothesize that such discrepancy could arise from the genotypic variance and the microenvironment interventions involving the physical, chemical, and biological parameters. Theoretical elucidation showed 3D-cultures exhibit larger total surface area (>78.5410-6 cm2), larger effective surface area available for mass transport (>52.3610-6 cm2 per cell), and larger contact surface area for anchorage (>26.1810-6 cm2). The 3-D and not the 2-D culture always faces a rotating force 3.10710-9 N/cell and a weaker gravitational force,  6.7710-7N/cell. As contrast, the 2-D cultured cells uniquely encounter a static hydraulic pressure 37.51 N/cm2. Kinetic analysis indicated 2-D culture mostly is accompanied with diffusion control over the substrate, contrasting with the kinetic control of 3-D cultures. For note, in principle severe cytotoxicity or mutagenicity only can occur at higher level toxicant under a kinetic control regime.
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How to cite this article:
Yeh Chen, Xian-Yu Yeh, Chiung-Chi Peng, Kuna-Chou Chen, Robert Y. Peng . Mathematical modeling predicts physical parameters which are pertinently affecting the 2D- and 3D- culture microenvironments. International Journal of Multidisciplinary Research and Development, Volume 1, Issue 5, 2014, Pages 05-17
International Journal of Multidisciplinary Research and Development International Journal of Multidisciplinary Research and Development