In vitro plant regeneration from mature embryos of wheat (Triticum aestivum L.)
M. M. Islam, R. N. Remme, D. Biswas, F. Yesmin
The present study was conducted at Plant Breeding and Biotechnology Laboratory, Agrotechnology Discipline, Khulna University, Khulna during February 2014 to December 2014 with a view to investigate the genotypic effect of 16 Bangladeshi wheat varieties on callus formation from mature seed embryos and followed by plant regeneration. MS basal salts with 3 mgL-1 2,4-D and 0.5 mgL-1 Kn was investigated for callus and hormone free MS media was selected for plant regeneration. Results of the experiment revealed that callus frequencies were significantly influenced with the wheat genotypes and callusing rate varied from 0 to 88%. Wheat genotype Aghrani, Bijoy, Sufi, Sonalika, Sourav, Barkot, Kolyansona, Prodip and Kheri did not respond at all to callus induction. Maximum and statistically highest callus frequency was noticed with the variety Pavon-76 followed by BARI gom 25 (68%), Akbar (64%) and Kanchan (28%) respectively. Study on morphogenesis of plated calli also showed variable results (0-61%). Whole plant regeneration ranged from 0 to 26%. Maximum regenerated plantlets obtained from the calli of the variety BARI gom 21 followed by BARI gom 25 (19%), Akbar (15%) and Pavon-76 (13%) respectively. Among the 16 wheat genotypes, variety Pavon-76 responded best in callusing whereas BARI gom 21 performed better in whole plant regeneration.