Development and validation of RP-UPLC method for the simultaneous estimation of pregabalin and epalrestat in pharmaceutical dosage form
Nagaraju Pappula, T Pradeep Kumar
In our present investigation we have developed an accurate procedure to estimate pregabalin and epalrestat in tablet dosage form. Chromatogram column was used HSS (100mmx2.1mm, 1.8m). Orthophosphoric acid (0.1%): acetonitrile 65:35 v/v was used as mobile phase and it was pumped through at a flow rate of 0.5 ml/min. Temperature was constant at 25°C. Wavelength was selected at 210 nm. Chromatographic retention time of pregabalin and epalrestat were observed at 1.100 min and 1.841 min. Percentage of recovery was acquired as100.25% and 100.68% for pregabalin and epalrestat. Limit of Detection (LOD) were found 0.02 and 0.11 respectively for pregabalin and epalrestat and limit of Quantitation (LOQ) were found 0.06 and 0.33 for pregabalin and epalrestat respectively. Results of analysis were validated statistically. The results of the study showed that the proposed RP-UPLC method is simple, rapid, precise and accurate, which is useful for the routine determination of pregabalin and epalrestat in tablet dosage form.